ELISpot Flex: Equine IL-10 (ALP)

ELISpot Flex: Equine IL-10 (ALP)

3126-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT3515
Detection mAbMT32AD, biotin
Enzyme conjugateStreptavidin-ALP

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Delivery 4-9 business days

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ELISpot substrate: BCIP/NBT-plus for ALP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting equine IL-10 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

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Product details

ProductELISpot Flex: Equine IL-10 (ALP)
ApplicationELISpot
AnalyteIL-10
ReactivityHorse
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of equine (horse) IL-10.
Cross-reactivityThe mAbs react with monkey IL-10 and cross-react with IL-10 from human and horse. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
PBMC from horse (50,000 cells/well) were incubated overnight with LPS or medium only and the number of cells secreting IL-10 was analyzed by ELISpot.
Datasheet/Protocol
SDS

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IL-10

Analyte description

Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.

Alternative namesInterleukin-10, IL-10, IL10
Cell typeT cell, Treg, Monocyte/MΦ, mDC
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ELISpot Plus: Equine IL-10 (ALP)